FOXE3 Antibody (PACO56394)
- SKU:
- PACO56394
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | FOXE3 Antibody (PACO56394) |
| Antibody SKU: | PACO56394 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
| Species Reactivity: | Human, Rat, Mouse |
| Immunogen: | Recombinant Human Forkhead box protein E3 protein (4-74AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Mouse brain tissue, Rat brain tissue. All lanes: FOXE3 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34 kDa. Observed band size: 34 kDa. |
 | Immunofluorescence staining of HepG2 cells with PACO56394 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Transcription factor that controls lens epithelial cell growth through regulation of proliferation, apoptosis and cell cycle. During lens development, controls the ratio of the lens fiber cells to the cells of the anterior lens epithelium by regulating the rate of proliferation and differentiation (By similarity). Controls lens vesicle closure and subsequent separation of the lens vesicle from ectoderm (By similarity). Controls the expression of DNAJB1 in a pathway that is crucial for the development of the anterior segment of the eye. |
| Synonyms: | Forkhead box protein E3 (Forkhead-related protein FKHL12) (Forkhead-related transcription factor 8) (FREAC-8), FOXE3, FKHL12 FREAC8 |
| UniProt Protein Function: | FOXE3: Defects in FOXE3 are a cause of anterior segment mesenchymal dysgenesis (ASMD); also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis. Defects in FOXE3 are a cause of congenital primary aphakia (CPA). Aphakia is a rare congenital eye disorder in which the lens is missing. It has been histologically subdivided into primary and secondary forms, in accordance with the severity of defects of the ocular tissues, whose development requires the initial presence of a lens. CPA results from an early developmental arrest, around the 4th-5th week of gestation in humans, that prevents the formation of any lens structure and leads to severe secondary ocular defects, including a complete aplasia of the anterior segment of the eye. In contrast, in secondary aphakic eyes, lens induction has occurred, and the lens vesicle has developed to some degree but finally has progressively resorbed perinatally, leading, therefore, to less-severe ocular defects. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1p32 Cellular Component: nucleus; transcription factor complex Molecular Function:DNA binding; sequence-specific DNA binding; transcription factor activity Biological Process: anatomical structure morphogenesis; cell development; cell differentiation; lens development in camera-type eye; negative regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter Disease: Anterior Segment Mesenchymal Dysgenesis; Aphakia, Congenital Primary |
| NCBI Summary: | This intronless gene belongs to the forkhead family of transcription factors, which is characterized by a distinct forkhead domain. The protein encoded functions as a lens-specific transcription factor and plays an important role in vertebrate lens formation. Mutations in this gene are associated with anterior segment mesenchymal dysgenesis and congenital primary aphakia. [provided by RefSeq, Dec 2009] |
| UniProt Code: | Q13461 |
| NCBI GenInfo Identifier: | 12644406 |
| NCBI Gene ID: | 2301 |
| NCBI Accession: | Q13461.2 |
| UniProt Secondary Accession: | Q13461,Q5SVY9, Q9NQV9, |
| UniProt Related Accession: | Q13461 |
| Molecular Weight: | 33,234 Da |
| NCBI Full Name: | Forkhead box protein E3 |
| NCBI Synonym Full Names: | forkhead box E3 |
| NCBI Official Symbol: | FOXE3Â Â |
| NCBI Official Synonym Symbols: | FKHL12; FREAC8Â Â |
| NCBI Protein Information: | forkhead box protein E3 |
| UniProt Protein Name: | Forkhead box protein E3 |
| UniProt Synonym Protein Names: | Forkhead-related protein FKHL12; Forkhead-related transcription factor 8; FREAC-8 |
| Protein Family: | Forkhead box protein |
| UniProt Gene Name: | FOXE3Â Â |
| UniProt Entry Name: | FOXE3_HUMAN |
| Antibodies | ELISA Kits |
| FOXE3 Antibody (PACO02744) | FOXE3 Colorimetric Cell-Based ELISA |
| FOXE3 Antibody (PACO09335) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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