The FOS Polyclonal Antibody (CAB17351) is a valuable tool for research involving the FOS protein, a transcription factor that plays a key role in cell proliferation, differentiation, and transformation. Raised in rabbits, this antibody is highly specific to human FOS samples and has been validated for use in a variety of applications, including Western blotting.FOS is a crucial component in the regulation of gene expression and is involved in various cellular processes such as cell growth, survival, and differentiation. Dysregulation of FOS has been linked to diseases like cancer, making it an important target for research in oncology and cell biology.
By specifically binding to the FOS protein, this antibody allows for the detection and analysis of FOS expression in different cell types, providing researchers with valuable insights into the role of FOS in cellular function and disease development. Its high reactivity and specificity make it an ideal tool for studies aiming to understand the molecular mechanisms underlying various pathologies and for the development of potential therapeutic strategies.
Product Name:
FOS Rabbit Polyclonal Antibody
SKU:
CAB17351
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FOS (NP_005243.1).
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Purification Method:
Affinity purification
Gene ID:
2353
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FOS antibody (CAB17351) at 1:1000 dilution.NIH/3T3 cells were treated by Serum-starvation overnight at 37℃.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
