The c-Fos Antibody (CAB0236) is a high-quality antibody developed for reliable detection and analysis of target proteins. The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. RRID AB_2757049 Gene ID 2353 Swiss Prot Synonym p55; AP-1; C-FOS; c-Fos
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
c-Fos Antibody
SKU:
CAB0236
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:5000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p55, AP-1, C-FOS, c-Fos
Positive Sample:
HeLa, HeLa treated with TPA, HeLa treated with TPA and starvation
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. RRID AB_2757049 Gene ID 2353 Swiss Prot Synonym p55; AP-1; C-FOS; c-Fos
Purification Method:
Affinity purification
Gene ID:
2353
RRID:
AB_2757049
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using c-Fos Rabbit pAb (CAB0236) at 1:1000 dilution. HeLa cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from HeLa cells, using c-Fos Rabbit pAb (CAB0236) at 1:1000 dilution. HeLa cells were treated with PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg c-Fos antibody (CAB0236). Western blot was performed from the immunoprecipitate using c-Fos antibody (CAB0236) at a dilution of 1:1000.
