The FMO5 Polyclonal Antibody (CAB7673) is a vital tool for research focused on FMO5, a member of the flavin-containing monooxygenase family involved in drug metabolism and detoxification. This antibody, generated in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for use in Western blot applications. By targeting the FMO5 protein, researchers can effectively detect and analyze its expression in various cell types, facilitating studies in pharmacology and toxicology.
FMO5, a key enzyme in the oxidation of xenobiotics and endogenous compounds, plays a crucial role in maintaining homeostasis and eliminating foreign substances from the body. Understanding the function and regulation of FMO5 is essential for investigating drug metabolism, drug-drug interactions, and toxicology. This antibody serves as a valuable tool for unraveling the molecular mechanisms underlying these processes, paving the way for the development of safer and more effective therapeutics.
Product Name:
FMO5 Rabbit Polyclonal Antibody
SKU:
CAB7673
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human FMO5 (NP_001138302.1).
Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2330
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FMO5 antibody (CAB7673) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
