The FMO3 Polyclonal Antibody (CAB1901) is a valuable tool for researchers studying FMO3, a key enzyme involved in the metabolism of xenobiotics and drugs in the liver. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications.FMO3, also known as flavin-containing monooxygenase 3, plays a crucial role in the detoxification of various compounds, including drugs, environmental toxins, and dietary components. Dysregulation of FMO3 activity has been linked to several metabolic disorders, making it a target of interest in pharmacology and toxicology research.
By specifically binding to the FMO3 protein, this antibody enables researchers to detect and analyze its expression levels in different cell types and tissues. Its applications extend to studies on drug metabolism, toxicology, and the development of therapeutic interventions for metabolic diseases. The FMO3 Polyclonal Antibody is a valuable tool for advancing our understanding of FMO3 function and its implications in human health and disease.
Product Name:
FMO3 Rabbit Polyclonal Antibody
SKU:
CAB1901
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 263-532 of human FMO3 (NP_001002294.1).
Flavin-containing monooxygenases (FMO) are an important class of drug-metabolizing enzymes that catalyze the NADPH-dependent oxygenation of various nitrogen-,sulfur-, and phosphorous-containing xenobiotics such as therapeutic drugs, dietary compounds, pesticides, and other foreign compounds. The human FMO gene family is composed of 5 genes and multiple pseudogenes. FMO members have distinct developmental- and tissue-specific expression patterns. The expression of this FMO3 gene, the major FMO expressed in adult liver, can vary up to 20-fold between individuals. This inter-individual variation in FMO3 expression levels is likely to have significant effects on the rate at which xenobiotics are metabolised and, therefore, is of considerable interest to the pharmaceutical industry. This transmembrane protein localizes to the endoplasmic reticulum of many tissues. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Mutations in this gene cause the disorder trimethylaminuria (TMAu) which is characterized by the accumulation and excretion of unmetabolized trimethylamine and a distinctive body odor. In healthy individuals, trimethylamine is primarily converted to the non odorous trimethylamine N-oxide.
Purification Method:
Affinity purification
Gene ID:
2328
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse liver, using FMO3 antibody (CAB1901) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
