The FMO1 Polyclonal Antibody (PACO44477) offered by AssayGenie is a vital tool for researchers studying FMO1, an enzyme involved in drug metabolism and detoxification processes in the liver and other tissues. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in a variety of applications, including Western blotting. By specifically binding to the FMO1 protein, this antibody allows for the detection and analysis of FMO1 expression in different cell types, making it a valuable asset for studies in pharmacology, toxicology, and liver disease research.
FMO1 is a key player in the biotransformation of xenobiotics and endogenous compounds, contributing to the elimination of potentially harmful substances from the body. Its involvement in drug metabolism pathways and detoxification mechanisms underscores its importance in understanding drug efficacy, toxicity, and drug-drug interactions. Research on FMO1 function and regulation is crucial for the development of safer and more effective therapeutic approaches in the pharmaceutical industry and clinical practice.
Antibody Name:
FMO1 Antibody (PACO44477)
Antibody SKU:
PACO44477
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Dimethylaniline monooxygenase [N-oxide-forming] 1 protein (363-532AA)
Form:
Liquid
Storage Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method:
Antigen Affinity Purified
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry of paraffin-embedded human colon cancer using PACO44477 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human gastric cancer using PACO44477 at dilution of 1:100.
Background:
This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Form I catalyzes the N-oxygenation of secondary and tertiary amines.
FMO1: This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Form I catalyzes the N-oxygenation of secondary and tertiary amines. Belongs to the FMO family.
UniProt Protein Details:
Protein type:EC 1.14.13.8; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450
Chromosomal Location of Human Ortholog: 1q24.3
Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen; integral to membrane
Biological Process: organic acid metabolic process; toxin metabolic process; xenobiotic metabolic process; response to lipopolysaccharide; response to osmotic stress; drug metabolic process
NCBI Summary:
Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
