The FLT3 Polyclonal Antibody (CAB7897) is a valuable tool for researchers studying FLT3, a cell surface receptor protein that plays a key role in the regulation of hematopoiesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the FLT3 protein, this antibody enables researchers to detect and analyze FLT3 expression in various cell types, making it an ideal choice for studies in hematology and oncology.
FLT3, also known as Fms-like tyrosine kinase 3, is a crucial factor in the development and function of hematopoietic stem cells. Mutations in the FLT3 gene have been associated with various hematological malignancies, highlighting its importance as a therapeutic target in cancer research. This antibody facilitates the study of FLT3 signaling pathways and the role of FLT3 in leukemogenesis, providing valuable insights for the development of targeted therapies for leukemia and other blood disorders.
Product Name:
FLT3 Rabbit Polyclonal Antibody
SKU:
CAB7897
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-270 of human FLT3 (NP_004110.2).
Endoplasmic reticulum lumen, Membrane, Single-pass type I membrane protein.
Calculated MW:
113kDa
Observed MW:
160kDa
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Purification Method:
Affinity purification
Gene ID:
2322
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Mouse liver, using FLT3 antibody (CAB7897) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
