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Anti-Ambra1 Antibody (CAB1083)

SKU:
CAB1083
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
  • Autophagy Antibodies Anti-Ambra1 Antibody CAB1083
  • Autophagy Antibodies Anti-Ambra1 Antibody CAB1083
  • Autophagy Antibodies Anti-Ambra1 Antibody CAB1083
€139 - €419
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Description

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Antibody Name:Anti-Ambra1 Antibody
Antibody SKU:CAB1083
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HeLa, Jurkat, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GAAH TSSP QPST SRGL LPEA GQLA ERGL SPRT ASWD QPGT PGRE PTQP TLPS SSPV PIPV SLPS AEGP TLHC ELTN NNHL LDGG SSRG DAAG PRGE PRNR
Gene ID:55626
Uniprot:Q9C0C7
Cellular Location:Cytoplasmic vesicle, autophagosome
Calculated MW:84kDa/132kDa/135kDa/139kDa/142kDa
Observed MW:143kDa
Synonyms:AMBRA1, DCAF3, WDR94, FLJ20294
Background:
UniProt Protein Function:AMBRA1: a WD40 protein that regulates autophagy and development of the nervous system. A highly conserved vertebrate protein whose expression is mostly confined to the neuroepithelium during early neurogenesis. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. Interacts with ATG6. Forms a complex with ATG6 and PIK3C3. Its functional inactivation in mouse is lethal in utero (starting from embryonic day 14.5), leading to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, and excessive apoptosis. Hyperproliferation followed by a wave of caspase-dependent cell death was the earliest detectable abnormality in the developing neuroepithelium. Five isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Autophagy; Vesicle

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: mitochondrial outer membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm; autophagic vacuole; phagocytic vesicle; axoneme; cytosol

Molecular Function:protein binding; ubiquitin protein ligase binding

Biological Process: neural tube development; negative regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; autophagy; negative regulation of neuron apoptosis; cell differentiation

UniProt Code:Q9C0C7
NCBI GenInfo Identifier:166215833
NCBI Gene ID:55626
NCBI Accession:Q9C0C7.2
UniProt Secondary Accession:Q9C0C7,A2AH22,
UniProt Related Accession:Q9C0C7
Molecular Weight:
NCBI Full Name:Activating molecule in BECN1-regulated autophagy protein 1
NCBI Synonym Full Names:autophagy and beclin 1 regulator 1
NCBI Official Symbol:AMBRA1  
NCBI Official Synonym Symbols:DCAF3; WDR94  
NCBI Protein Information:activating molecule in BECN1-regulated autophagy protein 1
UniProt Protein Name:Activating molecule in BECN1-regulated autophagy protein 1
Protein Family:Activating molecule in BECN1-regulated autophagy protein
UniProt Gene Name:AMBRA1  
UniProt Entry Name:AMRA1_HUMAN
Anti-Ambra1 Antibody (CAB1083)Western blot analysis of extracts of various cell lines, using Ambra1 Antibody (CAB1083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-Ambra1 Antibody (CAB1083)Immunohistochemistry of paraffin-embedded rat lung using AMBRA1 antibody (CAB1083) at dilution of 1:100 (40x lens).
Anti-Ambra1 Antibody (CAB1083)Immunohistochemistry of paraffin-embedded mouse lung using AMBRA1 antibody (CAB1083) at dilution of 1:100 (40x lens).

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