The FLC Polyclonal Antibody is a valuable tool for researchers studying immune regulation and immune responses. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the FLC protein, this antibody enables detection and analysis in a variety of cell types, making it ideal for studies in immunology and cancer research.FLC, also known as an immune regulator, plays a key role in maintaining immune homeostasis and modulating inflammatory responses. Its involvement in immune system function makes it a crucial target for research into diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.
Understanding the role of FLC is essential for the development of therapies that can manipulate or enhance immune responses in these disease contexts.Overall, the FLC Polyclonal Antibody is a valuable tool for researchers looking to investigate the role of FLC in immune regulation and disease pathology. Its high reactivity and specificity make it a reliable choice for immunology and cancer research studies.
Product Name:
FLC Polyclonal Antibody
SKU:
CAB21962
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-196 of arabidopsis thaliana FLC (NP_196576.1).
MADS-box protein encoded by FLOWERING LOCUS C - transcription factor that functions as a repressor of floral transition and contributes to temperature compensation of the circadian clock. Expression is downregulated during cold treatment. Vernalization, FRI and the autonomous pathway all influence the state of FLC chromatin. Both maternal and paternal alleles are reset by vernalization, but their earliest activation differs in timing and location. Histone H3 trimethylation at lysine 4 and histone acetylation are associated with active FLC expression, whereas histone deacetylation and histone H3 dimethylation at lysines 9 and 27 are involved in FLC repression. Expression is also repressed by two small RNAs (30- and 24-nt) complementary to the FLC sense strand 3? to the polyA site. The small RNAs are most likely derived from an antisense transcript of FLC. Interacts with SOC1 and FT chromatin in vivo. Member of a protein complex.
Purification Method:
Affinity purification
Gene ID:
830878
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using FLC Rabbit pAb (CAB21962) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.5s.
