The Flap Endonuclease 1 Polyclonal Antibody (CAB1175) is a valuable tool for research in DNA repair and replication processes. This antibody, created by Assay Genie, is specifically designed to target Flap Endonuclease 1 (FEN-1), a key enzyme involved in DNA metabolism.Raised in rabbits, this polyclonal antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the FEN-1 protein, researchers can accurately detect and analyze its expression levels in various cell types. This makes the CAB1175 antibody ideal for studies in molecular biology, genetics, and cancer research.
FEN-1 is a crucial enzyme that plays a role in the removal of flap structures during DNA replication and repair. Dysregulation of FEN-1 has been linked to genomic instability, a hallmark of cancer development. By studying the activity of FEN-1 using this antibody, researchers can gain valuable insights into the mechanisms underlying DNA damage and repair pathways.Overall, the Flap Endonuclease 1 Polyclonal Antibody (CAB1175) offers researchers a powerful tool for investigating the intricate processes of DNA metabolism and their implications in various disease states.
Product Name:
FEN-1 Rabbit Polyclonal Antibody
SKU:
CAB1175
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN-1 (NP_004102.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:10 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
MF1; RAD2; FEN-1
Positive Sample:
NIH/3T3,HeLa,Jurkat,Y79,Mouse spleen,Mouse testis
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion, Nucleus, nucleolus, nucleoplasm.
Calculated MW:
43kDa
Observed MW:
48kDa
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Purification Method:
Affinity purification
Gene ID:
2237
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using FEN-1 Rabbit pAb (CAB1175) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
