FEN-1 Rabbit Polyclonal Antibody (CAB1175)
- SKU:
- CAB1175
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | FEN-1 Rabbit Polyclonal Antibody |
SKU: | CAB1175 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN-1 (NP_004102.1). |
Sequence: | GDVL QNEE GETT SHLM GMFY RTIR MMEN GIKP VYVF DGKP PQLK SGEL AKRS ERRA EAEK QLQQ AQAA GAEQ EVEK FTKR LVKV TKQH NDEC KHLL SLMG IPYL DAPS EAEA SCAA LVKA GKVY AAAT EDMD CLTF GSPV LMRH LTAS EAKK LPIQ EFHL SRIL QELG LNQE QFVD LCIL LGSD YCES IRGI GPKR AVDL IQKH KSIE EIVR RLDP NKYP VPEN WLHK EAHQ LFLE PEVL DPES VELK WSEP NEEE LIKF MCGE KQFS EERI RSGV KRLS KSRQ GSTQ GRLD DFFK VTGS LSSA KRKE PEPK GSTK KKAK TGAA GKFK RGK |
Tested Applications: | WB IHC-P IF/ICC IP ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:10 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | MF1; RAD2; FEN-1 |
Positive Sample: | NIH/3T3,HeLa,Jurkat,Y79,Mouse spleen,Mouse testis |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion, Nucleus, nucleolus, nucleoplasm. |
Calculated MW: | 43kDa |
Observed MW: | 48kDa |
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Purification Method: | Affinity purification |
Gene ID: | 2237 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |