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FLAG-HRP Monoclonal Antibody [PFLAGHRPSHG] (CPAB0485)

SKU:
CPAB0485
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Universal
Host Species:
Mouse
Isotype:
IgG2b
Clone:
PFLAGHRPSHG
Applications:
WB
€726
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Description

system_update_alt Datasheet

FLAG-HRP Monoclonal Antibody [PFLAGHRPSHG] (CPAB0485)

The Flag-HRP Antibody (CPAB0485) is a powerful tool for researchers studying protein expression and localization of Flag-tagged proteins. This antibody, produced in rabbits, is highly specific and reactive with Flag-tag sequences, making it ideal for use in various applications such as Western blotting and immunohistochemistry.The Flag-HRP Antibody binds specifically to the Flag epitope, allowing for precise detection and visualization of Flag-tagged proteins in cell lysates or tissue samples. Its high sensitivity and specificity make it a valuable tool for studying protein expression levels, protein-protein interactions, and subcellular localization in a wide range of research settings.

Whether you are investigating protein function, protein-protein interactions, or signaling pathways, the Flag-HRP Antibody is a versatile and reliable choice for your research needs. With its exceptional performance and ease of use, this antibody is an essential tool for researchers exploring the intricate dynamics of protein biology.

Product Name:FLAG-HRP Antibody
Product Sku:CPAB0485
Size:100μl
Host Species:Mouse
Immunogen:
Clone:PFLAGHRPSHG.
Reactivity:Other bodies
Applications:Western Blot
Purification Method:
Isotype:IgG2b
Background:A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
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