The FKRP Polyclonal Antibody (CAB10194) is a valuable tool for researchers studying the FKRP protein, which is involved in the glycosylation of alpha-dystroglycan and plays a crucial role in muscular dystrophy. The antibody is produced in rabbits and has high reactivity with human samples, making it suitable for Western blot applications. By binding specifically to the FKRP protein, this antibody enables the detection and analysis of FKRP in various cell types, providing insights into its function and potential therapeutic implications.
FKRP is a key player in maintaining the integrity of muscle cells and defects in the protein have been linked to various forms of muscular dystrophy. Research on FKRP is essential for understanding the mechanisms underlying these debilitating disorders and identifying potential treatment targets. The FKRP Polyclonal Antibody offers a reliable method for investigating the expression and localization of FKRP in both healthy and diseased tissues, making it a valuable tool for studies in muscle biology and therapeutic development.
Product Name:
FKRP Rabbit Polyclonal Antibody
SKU:
CAB10194
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-220 of human FKRP (NP_077277.1).
Cell membrane, Golgi apparatus membrane, Rough endoplasmic reticulum, Secreted, Single-pass type II membrane protein, sarcolemma.
Calculated MW:
55kDa
Observed MW:
54kDa
This gene encodes a protein which is targeted to the medial Golgi apparatus and is necessary for posttranslational modification of dystroglycan. Mutations in this gene have been associated with congenital muscular dystrophy, cognitive disability, and cerebellar cysts. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
79147
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Rat brain, using FKRP antibody (CAB10194) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
