The FITC Rat IgG2b Isotype Control (LTF-2) is a critical tool for flow cytometry experiments, allowing researchers to distinguish non-specific binding from true positive signals. This isotype control, compatible with rat samples, is specifically designed to match the rat IgG2b antibody properties, providing a baseline reference for comparison in immunostaining procedures. By utilizing the FITC-conjugated IgG2b Isotype Control, researchers can accurately assess the specificity of their primary antibodies and ensure reliable and reproducible results in flow cytometry analyses.The FITC Rat IgG2b Isotype Control (LTF-2) serves as an essential negative control in experimental setups, helping to eliminate background noise and verify the specificity of staining.
With its consistent performance and high signal-to-noise ratio, this isotype control is a valuable instrument for researchers in immunology, cell biology, and related fields. By incorporating the FITC Rat IgG2b Isotype Control (LTF-2) into their experimental workflows, scientists can enhance the reliability and accuracy of their flow cytometry studies, leading to more robust data interpretation and meaningful discoveries in cellular and molecular research.
Product Name:
FITC Rat IgG2b, κ Isotype Control [LTF-2] (AGEL2666)
Product Code:
AGEL2666
Clone No:
LTF-2
Host Species:
Rat
Applications:
FCM
Isotype:
Rat IgG2b, κ
Conjugation:
FITC
Form:
Liquid
Fluorescent Label:
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.