The FITC Rat IgG2a Isotype Control (B39-4) is a vital tool for researchers working with fluorescence-based assays and immunostaining experiments. This isotype control antibody is specifically designed for use in flow cytometry, immunocytochemistry, and other fluorescence detection techniques where it serves as a crucial control to ensure specificity and accuracy of staining.As a rat IgG2a isotype control, B39-4 is ideal for determining non-specific binding of antibodies to cells or tissues in various experimental settings. By providing a reference standard for background fluorescence levels, researchers can confidently differentiate true positive signals from noise and artifacts in their samples.
This is particularly important in multi-color flow cytometry experiments and immunofluorescence studies where precise detection and quantification of specific targets are essential.The FITC-conjugated formulation of this isotype control offers the advantage of easy visualization and compatibility with commonly used fluorescence detection systems. Its high specificity and low background staining make it a reliable choice for ensuring the accuracy and reliability of experimental results in diverse research applications. If you require a dependable control for your fluorescence-based assays, the FITC Rat IgG2a Isotype Control (B39-4) is an indispensable reagent to support your research efforts.
Product Name:
FITC Rat IgG2a, λ Isotype Control [B39-4] (AGEL2892)
Product Code:
AGEL2892
Clone No:
B39-4
Host Species:
Rat
Applications:
FCM
Isotype:
Rat IgG2a, λ
Conjugation:
FITC
Form:
Liquid
Fluorescent Label:
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.