The FITC Mouse IgG3 Isotype Control (A112-3) is an essential tool for flow cytometry experiments requiring precise immunophenotyping and antibody specificity validation. This isotype control antibody, manufactured by Assay Genie, is specifically designed as a negative control to assess non-specific background staining in flow cytometry assays, ensuring accurate interpretation of results.Flow cytometry is a powerful technique used in immunology, oncology, and other fields to analyze and quantify cell populations based on specific surface markers. The FITC Mouse IgG3 Isotype Control (A112-3) serves as a reference standard for flow cytometry experiments involving mouse IgG3 primary antibodies, helping researchers distinguish true antigen-specific staining from non-specific binding.
With its high purity, consistency, and specificity, this isotype control antibody is an ideal choice for researchers conducting multi-color flow cytometry experiments where precise antibody labeling and signal detection are critical. Whether investigating immune cell subsets, examining protein expression profiles, or characterizing cellular interactions, the FITC Mouse IgG3 Isotype Control (A112-3) from Assay Genie provides confidence and reliability in flow cytometry data analysis.
Product Name:
FITC Mouse IgG3, κ Isotype Control [A112-3] (AGEL2917)
Product Code:
AGEL2917
Clone No:
A112-3
Host Species:
Mouse
Applications:
FCM
Isotype:
Mouse IgG3, κ
Conjugation:
FITC
Form:
Liquid
Fluorescent Label:
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.