The FITC Mouse IgG2a Isotype Control (C1.18.4) is a vital tool for immunological research, providing a baseline for comparison in flow cytometry and other assays. This isotype control antibody, sourced from mouse IgG2a, allows researchers to distinguish specific staining from non-specific background signals, ensuring the accuracy of their results. It is essential for validating the specificity of primary antibodies binding to cell surface markers and can be used across a range of cell types and experimental conditions.In flow cytometry experiments, the FITC Mouse IgG2a Isotype Control (C1.
18.4) serves as a crucial negative control to assess the level of background fluorescence and set appropriate gates for analysis. By using this control alongside experimental samples, researchers can confidently identify true positive signals and draw reliable conclusions from their data. This isotype control is particularly valuable in studies where precise quantification and characterization of cell populations are essential, such as immunophenotyping, cell cycle analysis, and receptor binding studies.
Product Name:
FITC Mouse IgG2a, κ Isotype Control [C1.18.4] (AGEL2585)
Product Code:
AGEL2585
Clone No:
C1.18.4
Host Species:
Mouse
Applications:
FCM
Isotype:
Mouse IgG2a, κ
Conjugation:
FITC
Form:
Liquid
Fluorescent Label:
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.