FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific (CABS083)
The FITC-conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (Fc Fragment Specific) (CABS083) is a versatile tool for researchers studying the immune response. This antibody, targeted against the Fc region of rabbit IgG, allows for specific labeling and detection of rabbit IgG in various applications such as immunofluorescence and flow cytometry.With its high sensitivity and specificity, this antibody is an essential reagent for studies investigating antibody-mediated immune responses and immune cell interactions.
The FITC conjugation enables easy visualization of target molecules, providing valuable insights into the dynamics of immune processes.Whether studying immune cell activation, antibody production, or immune complex formation, the FITC-conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (Fc Fragment Specific) (CABS083) offers reliable and accurate detection of rabbit IgG, facilitating precise and detailed analysis of immune-related phenomena. Trust this antibody for your immunology research needs.
Product Name:
FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
SKU:
CABS083
Size:
100uL
Isotype:
Fluorescein conjugated IgG
Host Species:
Goat
Immunogen:
Rabbit IgG
Tested Applications:
IF/ICCFC
Recommended Dilution:
IF/ICC,1:100 - 1:500 FC,1:50 - 1:200
Conjugate:
FITC. Ex:491nm. Em:516nm.
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol,pH7.3.
Confocal imaging of paraffin-embedded Mouse brain using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
