FITC-Conjugated Donkey Anti-Rabbit IgG (H+L) (CABS042) is a highly specific and sensitive secondary antibody designed for use in immunofluorescence and flow cytometry applications. This antibody is raised in donkeys and conjugated with the fluorescent dye FITC, providing excellent signal amplification for the detection of rabbit primary antibodies in various experimental settings.With its high affinity for rabbit IgG, this conjugated antibody allows for the accurate and reliable visualization of target antigens in cells and tissues. Its versatility and compatibility with a wide range of sample types make it an essential tool for researchers studying protein localization, biomarker expression, and cellular signaling pathways.
The FITC-Conjugated Donkey Anti-Rabbit IgG (H+L) (CABS042) antibody is an invaluable resource for immunologists, cell biologists, and cancer researchers seeking to explore and understand the intricate mechanisms of immune response modulation and protein detection. Its superior performance and specificity make it a valuable addition to any laboratory conducting cutting-edge research in the fields of immunology and molecular biology.
Product Name:
FITC-conjugated Donkey anti-Rabbit IgG (H+L)
SKU:
CABS042
Size:
100uL
Isotype:
Fluorescein conjugated IgG
Host Species:
Donkey
Immunogen:
Rabbit IgG
Tested Applications:
IF/ICCFC
Recommended Dilution:
IF/ICC,1:50 - 1:200 FC,1:50 - 1:200
Conjugate:
FITC. Ex:491nm. Em:516nm.
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol,pH7.3.
Immunofluorescence analysis of PC-12 cells using FITC Donkey Anti-Rabbit IgG (H+L) (CABS042) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
