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FITC/5-FAM/6-FAM Monoclonal Antibody (CAB22444)

SKU:
CAB22444
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $635
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:FITC/5-FAM/6-FAM Monoclonal Antibody
SKU:CAB22444
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Species independent
Immunogen:FITC
Sequence:FITC con juga ted to K LH
Tested Applications:WB ELISA
Recommended Dilution:ELISA,1:500 - 1:2000 WB,1:1000 - 1:2000
Conjugate:Unconjugated
Observed MW:25kDa/55kDa

Fluorescein isothiocyanate (FITC) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). 5-FAM is a single isomer with Ex/Em of 490 nm/520 nm. The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules.

Purification Method:Affinity purification
Clone Number:ARC51073
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageImmobilized biotin-6 Fam, biotin-5 Fam, biotin-FITC, at 1 μg/mL (25 μL/well), of which EC50ranges are 0.18-0.40 ng/mL, 0.19-0.29 ng/mL and 0.15-0.29 ng/mL ,can bind anti 6-Fam/ 5-Fam/ FITC mAb (CAB22444), respectively.

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