The FITC/5-FAM/6-FAM Monoclonal Antibody is a highly specific and sensitive tool for detecting and analyzing target molecules in various cellular applications. This antibody, conjugated to the popular fluorescent dyes FITC, 5-FAM, and 6-FAM, provides researchers with multiple options for visualization and quantification of their target of interest.With a strong signal-to-noise ratio and low nonspecific binding, this antibody is ideal for use in flow cytometry, immunofluorescence, and immunohistochemistry experiments. Its versatility and reliability make it a valuable tool for studying protein expression, localization, and interactions in both normal and diseased cells.
Whether investigating basic cellular functions or searching for potential diagnostic or therapeutic targets in diseases such as cancer, autoimmune disorders, or infectious diseases, the FITC/5-FAM/6-FAM Monoclonal Antibody offers researchers a high-quality reagent to advance their research goals. Trust in its performance and precision to enhance your studies in the fields of immunology, oncology, and beyond.
Product Name:
FITC/5-FAM/6-FAM Monoclonal Antibody
SKU:
CAB22444
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
FITC
Sequence:
FITC con juga ted to K LH
Tested Applications:
WBELISA
Recommended Dilution:
ELISA,1:500 - 1:2000 WB,1:1000 - 1:2000
Conjugate:
Unconjugated
Observed MW:
25kDa/55kDa
Fluorescein isothiocyanate (FITC) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). 5-FAM is a single isomer with Ex/Em of 490 nm/520 nm. The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules.
Purification Method:
Affinity purification
Clone Number:
ARC51073
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Immobilized biotin-6 Fam, biotin-5 Fam, biotin-FITC, at 1 μg/mL (25 μL/well), of which EC50ranges are 0.18-0.40 ng/mL, 0.19-0.29 ng/mL and 0.15-0.29 ng/mL ,can bind anti 6-Fam/ 5-Fam/ FITC mAb (CAB22444), respectively.
