The FIP1L1 Polyclonal Antibody (CAB7138) is a vital tool for researchers studying FIP1L1, a crucial protein involved in gene expression regulation. This antibody, generated in rabbits, exhibits high specificity and sensitivity when used with human samples, making it an excellent choice for Western blot applications. By targeting the FIP1L1 protein, researchers can accurately detect and study its function in different cell types, making it ideal for investigations in molecular biology and genetics.FIP1L1 is a key component in the formation of the pre-mRNA 3' processing complex, playing a critical role in gene transcription and RNA processing.
Dysregulation of FIP1L1 has been linked to various diseases, including cancer and genetic disorders. By understanding the mechanisms by which FIP1L1 functions, researchers can gain valuable insights into disease pathogenesis and potentially identify new therapeutic targets for treatment. Overall, the FIP1L1 Polyclonal Antibody (CAB7138) is a valuable resource for researchers looking to expand their knowledge of gene regulation and explore the implications of FIP1L1 in health and disease.
Product Name:
FIP1L1 Rabbit Polyclonal Antibody
SKU:
CAB7138
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human FIP1L1 (NP_001128409.1).
This gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. This gene, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly(A) polymerase activity. Its N-terminus contains a PAP-binding site and its C-terminus an RNA-binding domain. An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that joins the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion is the cause of some forms of idiopathic hypereosinophilic syndrome (HES). This syndrome, recently reclassified as chronic eosinophilic leukemia (CEL), is responsive to treatment with tyrosine kinase inhibitors. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
81608
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using FIP1L1 Rabbit pAb (CAB7138) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
