The FHL1 Polyclonal Antibody (CAB5460) is a valuable tool for researchers studying FHL1, a member of the four-and-a-half LIM domain protein family known for its role in muscle development and function. This antibody, generated in rabbits, exhibits high specificity for human samples and is validated for use in various applications, including Western blot analysis. By targeting the FHL1 protein, this antibody allows for accurate detection and analysis in different cell types, making it a versatile option for studies in muscle biology and related fields.
FHL1, also referred to as four and a half LIM domains 1, is involved in important cellular processes such as muscle cell differentiation, sarcomere assembly, and mechanotransduction. Dysregulation of FHL1 has been linked to various muscular disorders, making it a potential therapeutic target for conditions like muscular dystrophy and myopathies. Investigating the function and expression of FHL1 using this antibody can provide valuable insights into the mechanisms underlying these diseases and guide the development of targeted treatment strategies.
Product Name:
FHL1 Rabbit Polyclonal Antibody
SKU:
CAB5460
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-296 of human FHL1 (NP_001153171.1).
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
2273
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FHL1 antibody (CAB5460) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
