The FHIT Polyclonal Antibody (CAB1196) is a valuable tool for researchers investigating the FHIT gene, a tumor suppressor gene that is frequently lost or inactivated in various cancers. This antibody, produced in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples.The FHIT gene plays a critical role in maintaining genomic stability and protecting against cancer development. Loss of FHIT expression has been associated with a poor prognosis in multiple cancer types, making it a promising target for cancer research and therapy development.
By detecting the FHIT protein, researchers can analyze its expression levels and activity in different cell types, providing valuable insights into the mechanisms underlying cancer progression and potential therapeutic strategies. The FHIT Polyclonal Antibody is a reliable tool for studies in oncology and molecular biology, helping to further our understanding of cancer biology and potentially leading to the development of novel treatment options.
Product Name:
FHIT Rabbit Polyclonal Antibody
SKU:
CAB1196
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human FHIT (NP_001159715.1).
The protein encoded by this gene is a P1-P3-bis(5'-adenosyl) triphosphate hydrolase involved in purine metabolism. This gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. The encoded protein is also a tumor suppressor, as loss of its activity results in replication stress and DNA damage.
Purification Method:
Affinity purification
Gene ID:
2272
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FHIT antibody (CAB1196) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
