The FGD4 Polyclonal Antibody (CAB8596) is a valuable tool for research involving the FGD4 protein, which plays a crucial role in cell signaling pathways and actin cytoskeleton reorganization. This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for various research applications, including Western blotting.The FGD4 protein is a guanine nucleotide exchange factor that activates Rho GTPases, regulating cellular processes such as cell adhesion, migration, and cytoskeletal dynamics. Dysregulation of FGD4 expression or function has been implicated in diseases such as cancer, neurodevelopmental disorders, and cardiovascular conditions.
Therefore, studying the role of FGD4 in these contexts is essential for advancing our understanding of disease mechanisms and developing targeted therapies.By using the FGD4 Polyclonal Antibody, researchers can accurately detect and analyze FGD4 protein levels in different cell types and tissues, enabling deeper insights into its functions and interactions. This antibody is a valuable tool for investigators in the fields of cell biology, signal transduction, and disease research, facilitating the exploration of FGD4's role in health and disease.
Product Name:
FGD4 Rabbit Polyclonal Antibody
SKU:
CAB8596
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 99-198 of human FGD4 (NP_640334.2).
This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
121512
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FGD4 antibody (CAB8596) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
