FEZF1 Antibody (PACO60701)
- SKU:
- PACO60701
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | FEZF1 Antibody (PACO60701) |
| Antibody SKU: | PACO60701 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Fez family zinc finger protein 1 protein (1-115AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: U251 whole cell lysate. All lanes: FEZF1 antibody at 6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 47, 52 kDa. Observed band size: 53 kDa. |
 | IHC image of PACO60701 diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Transcription repressor. Involved in the axonal projection and proper termination of olfactory sensory neurons (OSN). Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation. Expression is required in OSN to cell-autonomously regulate OSN axon projections. Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. May be required for correct rostral migration of the interneuron progenitors (By similarity). |
| Synonyms: | Fez family zinc finger protein 1 (Zinc finger protein 312B), FEZF1, FEZ ZNF312B |
| UniProt Protein Function: | FEZF1: Transcription repressor. Involved in the axonal projection and proper termination of olfactory sensory neurons (OSN). Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation. Expression is required in OSN to cell-autonomously regulate OSN axon projections. Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. May be required for correct rostral migration of the interneuron progenitors. Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: C2H2-type zinc finger protein; Cell development/differentiation; Transcription factor; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 7q31.32Biological Process: axon guidance; negative regulation of transcription, DNA-dependentDisease: Hypogonadotropic Hypogonadism 22 With Or Without Anosmia |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a transcriptional repressor that belongs to the zinc finger double domain protein family. The encoded protein is thought to play a role in the embryonic migration of gonadotropin-releasing hormone neurons into the brain. Mutations in this gene are associated with hypogonadotropic hypogonadism-22 with anosmia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| UniProt Code: | A0PJY2 |
| NCBI GenInfo Identifier: | 152032459 |
| NCBI Gene ID: | 389549 |
| NCBI Accession: | A0PJY2.1 |
| UniProt Secondary Accession: | A0PJY2,A0PJY3, A4D0W3, B4DUP9, B7ZM98 |
| UniProt Related Accession: | A0PJY2 |
| Molecular Weight: | 51,651 Da |
| NCBI Full Name: | Fez family zinc finger protein 1 |
| NCBI Synonym Full Names: | FEZ family zinc finger 1 |
| NCBI Official Symbol: | FEZF1Â Â |
| NCBI Official Synonym Symbols: | FEZ; HH22; ZNF312BÂ Â |
| NCBI Protein Information: | fez family zinc finger protein 1 |
| UniProt Protein Name: | Fez family zinc finger protein 1 |
| UniProt Synonym Protein Names: | Zinc finger protein 312B |
| Protein Family: | Fez family zinc finger protein |
| UniProt Gene Name: | FEZF1Â Â |
| UniProt Entry Name: | FEZF1_HUMAN |