The FEN1 Rabbit Monoclonal Antibody (CAB8999) is a high-quality antibody specifically designed for research involving FEN1, a key enzyme involved in DNA replication, repair, and recombination processes. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. It binds specifically to the FEN1 protein, allowing for accurate detection and analysis in various cell types, making it ideal for studies in genetics, cancer research, and other areas of molecular biology.FEN1, also known as flap endonuclease 1, is essential for maintaining genomic stability by processing DNA intermediates during DNA replication and repair.
Dysregulation of FEN1 has been implicated in various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic intervention. The FEN1 Rabbit Monoclonal Antibody provides researchers with a powerful tool to study the function and regulation of FEN1, ultimately contributing to our understanding of DNA repair mechanisms and potential disease treatments.
Product Name:
FEN-1 Rabbit Monoclonal Antibody
SKU:
CAB8999
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human FEN-1 (P39748).
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Purification Method:
Affinity purification
Gene ID:
2237
Clone Number:
ARC1376
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using FEN-1 Rabbit mAb (CAB8999) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
