FEN-1 Rabbit Monoclonal Antibody (CAB8999)
- SKU:
- CAB8999
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | FEN-1 Rabbit Monoclonal Antibody |
SKU: | CAB8999 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human FEN-1 (P39748). |
Sequence: | IQKH KSIE EIVR RLDP NKYP VPEN WLHK EAHQ LFLE PEVL DPES VELK WSEP NEEE LIKF MCGE KQFS EERI RSGV KRLS KSRQ GSTQ GRLD DFFK VTGS L |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 |
Synonyms: | MF1; RAD2; FEN-1 |
Positive Sample: | 293T,HUVEC,Mouse thymus |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion, Nucleus, nucleolus, nucleoplasm. |
Calculated MW: | 43kDa |
Observed MW: | 45kDa |
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Purification Method: | Affinity purification |
Gene ID: | 2237 |
Clone Number: | ARC1376 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |