The FEN1 Polyclonal Antibody (PAC063543) is a valuable tool for researchers studying FEN1, a key enzyme involved in DNA replication and repair processes. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting FEN1 in human samples, making it ideal for use in Western blot applications.FEN1, also known as flap endonuclease 1, plays a crucial role in maintaining genomic stability by removing RNA primers during DNA synthesis and processing DNA structures. Dysregulation of FEN1 has been associated with various diseases, including cancer and neurodegenerative disorders, making it a target of interest for therapeutic interventions.
By using the FEN1 Polyclonal Antibody, researchers can investigate the expression levels and localization of FEN1 in different cell types and tissues, providing valuable insights into its function and potential implications in disease development. This antibody is a valuable tool for studies in molecular biology, oncology, and genetics, contributing to advancements in understanding DNA repair mechanisms and disease pathogenesis.
Antibody Name:
FEN1 Antibody (PACO63543)
Antibody SKU:
PACO63543
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:1000-1:5000
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant Arabidopsis thaliana Flap endonuclease 1 protein (85-383AA)
Western Blot. Positive WB detected in Recombinant protein. All lanes: FEN1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37 kDa. Observed band size: 45 kDa.
Background:
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinicapyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
