The FECH Polyclonal Antibody (CAB8418) is a high-quality research tool for investigating Ferrochelatase (FECH), an essential enzyme involved in heme biosynthesis. Raised in rabbits, this antibody is highly specific to FECH and is suitable for use in various applications, including Western blotting and immunohistochemistry.FECH plays a crucial role in catalyzing the final step of heme biosynthesis, converting protoporphyrin IX into heme. Dysregulation of FECH expression or activity has been associated with various disorders, including porphyrias and iron metabolism abnormalities. By using the FECH Polyclonal Antibody, researchers can study the expression and localization of FECH in different cell types and tissues, providing insights into its function and potential therapeutic implications.
This antibody is validated for use with human samples, offering researchers a reliable tool for investigating the role of FECH in health and disease. Whether studying heme metabolism, iron homeostasis, or related pathways, the FECH Polyclonal Antibody is a valuable resource for advancing scientific knowledge in the fields of biochemistry, metabolism, and molecular biology.
Product Name:
FECH Rabbit Polyclonal Antibody
SKU:
CAB8418
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FECH (NP_000131.2).
The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.
Purification Method:
Affinity purification
Gene ID:
2235
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using FECH Rabbit pAb (CAB8418) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
