The FCGR3A Polyclonal Antibody (CAB2552) is a valuable tool for researchers studying the FCGR3A protein, a cell surface receptor involved in immune responses, particularly in the context of antibody-dependent cell-mediated cytotoxicity (ADCC). This antibody is generated in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications.FCGR3A, also known as CD16a, plays a crucial role in antibody-mediated immune responses by binding to the Fc portion of immunoglobulins, thereby triggering cellular cytotoxicity.
Understanding the function and regulation of FCGR3A is essential for advancing research in areas such as immunotherapy, infectious diseases, and autoimmune disorders.The FCGR3A Polyclonal Antibody enables researchers to detect and analyze FCGR3A expression in various cell types, offering insights into its role in immune modulation and disease pathogenesis. By targeting FCGR3A, scientists can develop innovative strategies for manipulating immune responses and enhancing therapeutic outcomes in conditions where immune dysregulation is implicated.
Product Name:
FCGR3A Rabbit Polyclonal Antibody
SKU:
CAB2552
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human FCGR3A (NP_000560.7).
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
29kDa
Observed MW:
60kDa
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2214
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FCGR3A antibody (CAB2552) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
