The FBXO9 Polyclonal Antibody (PAC048794) is a crucial tool for researchers studying FBXO9, a protein involved in regulating cellular processes and protein turnover. This antibody, produced in rabbits, demonstrates high specificity for human samples and is validated for use in Western blot applications. By targeting the FBXO9 protein, this antibody enables precise detection and analysis in various cell types, making it an essential asset for research in molecular biology and cell signaling pathways.FBXO9 is known for its role in ubiquitin-mediated protein degradation, influencing the stability and activity of key regulatory proteins within the cell.
Dysfunction of FBXO9 has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular homeostasis and disease progression. The FBXO9 Polyclonal Antibody provides researchers with a reliable tool to investigate the function and mechanisms of FBXO9, ultimately paving the way for novel therapeutic strategies targeting this critical protein.
Immunohistochemistry of paraffin-embedded human pancreatic tissue using PACO48794 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO48794 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1.
Synonyms:
F-box only protein 9 (Cross-immune reaction antigen 1) (Renal carcinoma antigen NY-REN-57), FBXO9, FBX9 VCIA1
UniProt Protein Function:
FBXO9: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Ubiquitin conjugating system; LigaseChromosomal Location of Human Ortholog: 6p12.3-p11.2Cellular Component: cytoplasm; SCF ubiquitin ligase complex; ubiquitin ligase complexMolecular Function: protein binding; ubiquitin-protein ligase activityBiological Process: fat cell differentiation; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; innate immune response; protein ubiquitination; regulation of TOR signaling pathway
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates at least 3 transcript variants diverging at the 5' terminus. [provided by RefSeq, Jul 2008]
