The FBXO3 Polyclonal Antibody (CAB15813) is a valuable tool for researchers studying FBXO3, a key protein involved in cellular regulation and degradation pathways. This antibody, produced in rabbits, demonstrates high specificity and reactivity with human samples, making it an ideal choice for various research applications, including Western blot analysis.FBXO3 is a substrate recognition component of the Skp1-Cullin1-F-box protein (SCF) E3 ubiquitin ligase complex, playing a crucial role in the targeted degradation of specific cellular proteins. By targeting substrates for ubiquitination and subsequent degradation, FBXO3 contributes to the maintenance of cellular homeostasis and regulation of various cellular processes.
Understanding the function and regulation of FBXO3 is essential for unraveling its role in disease states, including cancer and neurodegenerative disorders. The FBXO3 Polyclonal Antibody provides researchers with a reliable tool to investigate the expression levels and localization of FBXO3 in different cell types and tissues, offering valuable insights into its molecular mechanisms and potential therapeutic applications.
Product Name:
FBXO3 Rabbit Polyclonal Antibody
SKU:
CAB15813
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human FBXO3 (NP_036307.2).
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end.
Purification Method:
Affinity purification
Gene ID:
26273
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FBXO3 Rabbit pAb (CAB15813) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
