The FBXL21 Polyclonal Antibody (PAC047230) is a valuable tool for researchers studying FBXL21, a protein involved in regulating cellular processes and protein turnover. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the FBXL21 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for investigations in cell biology and molecular biology research.FBXL21 is a member of the F-box protein family, known for its role in targeting specific proteins for degradation through the ubiquitin-proteasome system. It plays a crucial role in maintaining protein homeostasis and regulating various cellular functions, making it a potential target for therapeutic interventions in diseases related to protein dysregulation.
By studying the function of FBXL21, researchers can gain insights into pathways involved in disease pathology and identify novel strategies for targeted therapies.This FBXL21 Polyclonal Antibody is a valuable asset for researchers interested in elucidating the mechanisms underlying protein degradation and cellular signaling pathways. With its high specificity and sensitivity, this antibody enables precise detection of FBXL21 levels in various experimental settings, facilitating in-depth investigations into its biological functions and potential therapeutic applications.
Western blot. All lanes: FBL21 antibody at 10 µg/ml. Lane 1: Mouse heart tissue. Lane 2: Mouse skeletal muscle tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 49 kDa. Observed band size: 49 kDa..
Immunofluorescent analysis of HepG2 cells using PACO47230 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human lung cancer using PACO47230 at dilution of 1:100.
Background:
Substrate-recognition component of the SCF(FBXL21) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation. The SCF(FBXL21) complex mainly acts in the cytosol and mediates ubiquitination of CRY proteins (CRY1 and CRY2), leading to CRY proteins stabilization. The SCF(FBXL21) complex counteracts the activity of the SCF(FBXL3) complex and protects CRY proteins from degradation. Involved in the hypothalamic suprachiasmatic nucleus (SCN) clock regulating temporal organization of the daily activities (By similarity).
Synonyms:
F-box/LRR-repeat protein 21 (F-box and leucine-rich repeat protein 21) (F-box and leucine-rich repeat protein 3B) (F-box/LRR-repeat protein 3B), FBXL21, FBL21 FBL3 FBXL3B FBXL3P
UniProt Protein Function:
FBXL21: Substrate-recognition component of some SCF (SKP1-CUL1- F-box protein)-type E3 ubiquitin ligase complex involved in circadian pacemaker function. The SCF(FBXL21) complex acts by mediating ubiquitination and subsequent degradation of CRY1. Probable clock-controlled protein that plays a specific role in suprachiasmatic nucleus, SCN and pacemaker function.Chromosomal Location of Human Ortholog: 5q31Cellular Component: SCF ubiquitin ligase complex; cytosol; nucleus; ubiquitin ligase complexMolecular Function: ubiquitin-protein ligase activityBiological Process: rhythmic process; protein ubiquitination; entrainment of circadian clock by photoperiod
UniProt Protein Details:
NCBI Summary:
This locus represents a transcribed pseudogene that is related to genes encoding members of the F-box family of proteins. [provided by RefSeq, Nov 2017]
