The FBN1 Polyclonal Antibody (CAB16677) is a powerful tool for researchers studying the extracellular matrix protein fibrillin-1, encoded by the FBN1 gene. This antibody, produced in rabbits, is highly reactive with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.Fibrillin-1 is a key component of microfibrils in connective tissues, playing a critical role in maintaining the structural integrity of tissues and organs. Mutations in the FBN1 gene are associated with various genetic disorders, including Marfan syndrome, highlighting the importance of studying this protein in both normal and pathological conditions.
By specifically targeting Fibrillin-1, researchers can detect and analyze its expression in different cell types and tissues, providing valuable insights into its function and role in disease development. The FBN1 Polyclonal Antibody is a valuable tool for studies in molecular biology, genetics, and biomedical research, offering a deeper understanding of the complex mechanisms involving fibrillin-1.
Product Name:
FBN1 Rabbit Polyclonal Antibody
SKU:
CAB16677
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 360-460 of human Fibrillin 1 (NP_000129.3).
basement membrane, endoplasmic reticulum lumen, extracellular region, extracellular space
Calculated MW:
312kDa
Observed MW:
312kDa
This gene encodes a member of the fibrillin family of proteins. The encoded preproprotein is proteolytically processed to generate two proteins including the extracellular matrix component fibrillin-1 and the protein hormone asprosin. Fibrillin-1 is an extracellular matrix glycoprotein that serves as a structural component of calcium-binding microfibrils. These microfibrils provide force-bearing structural support in elastic and nonelastic connective tissue throughout the body. Asprosin, secreted by white adipose tissue, has been shown to regulate glucose homeostasis. Mutations in this gene are associated with Marfan syndrome and the related MASS phenotype, as well as ectopia lentis syndrome, Weill-Marchesani syndrome, Shprintzen-Goldberg syndrome and neonatal progeroid syndrome.
Purification Method:
Affinity purification
Gene ID:
2200
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat pancreas, using Fibrillin 1 Rabbit pAb (CAB16677) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human liver using Fibrillin 1 Rabbit pAb (CAB16677) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human testis using Fibrillin 1 Rabbit pAb (CAB16677) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Fibrillin 1 Rabbit pAb (CAB16677) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Fibrillin 1 Rabbit pAb (CAB16677) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
