The Fatty Acid Synthase Rabbit Monoclonal Antibody (CAB19050) is a cutting-edge tool for researchers studying fatty acid synthesis, a key metabolic process implicated in various diseases including cancer and metabolic disorders. This high-quality antibody, produced using rabbit monoclonal technology, demonstrates exceptional reactivity with human samples and is validated for use in applications such as Western blotting.Fatty acid synthase (FAS) is a vital enzyme involved in the de novo synthesis of fatty acids, which are essential components of cellular membranes and energy storage. Dysregulation of FAS has been linked to cancer progression, making it a promising therapeutic target in oncology research.
The CAB19050 antibody specifically binds to FAS, enabling precise detection and quantification in a variety of cell types and tissue samples.By utilizing the Fatty Acid Synthase Rabbit Monoclonal Antibody, researchers can gain deeper insights into the role of FAS in lipid metabolism, energy balance, and disease pathogenesis. This antibody is a valuable tool for studying the molecular mechanisms underlying FAS dysregulation and exploring potential therapeutic strategies for diseases associated with aberrant fatty acid synthesis.
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
FAS; OA-519; SDR27X1; Fatty Acid Synthase (FASN)
Positive Sample:
A549,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Melanosome.
Calculated MW:
273kDa
Observed MW:
273kDa
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Purification Method:
Affinity purification
Gene ID:
2194
Clone Number:
ARC0377
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Fatty Acid Synthase (FASN) Rabbit mAb (CAB19050) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
