The Fatty Acid Synthase Polyclonal Antibody (CAB0461) is a valuable tool for researchers studying fatty acid metabolism and related pathways. This antibody, raised in rabbits, is highly specific to human samples and is validated for use in Western blot applications. It binds specifically to the Fatty Acid Synthase protein, allowing for accurate detection and analysis in various cell types.Fatty Acid Synthase is a key enzyme involved in the de novo synthesis of long-chain fatty acids, a process essential for cellular function and energy storage. Dysregulation of fatty acid metabolism has been implicated in various diseases, including cancer, obesity, and metabolic disorders.
Research into the role of Fatty Acid Synthase in these conditions is crucial for developing targeted therapies and understanding disease progression.By using the Fatty Acid Synthase Polyclonal Antibody, researchers can gain valuable insights into the regulation and function of this enzyme, advancing our understanding of fatty acid metabolism and its impact on health and disease. This antibody is a valuable tool for studies in biochemistry, cell biology, and cancer research, offering researchers a reliable means to investigate the complex role of Fatty Acid Synthase in cellular processes.
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Purification Method:
Affinity purification
Gene ID:
2194
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Fatty Acid Synthase (FASN) Rabbit pAb (CAB0461) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
