The FAS Polyclonal Antibody (CAB12401) is a valuable tool for researchers studying the FAS protein, also known as CD95 or APO-1. This antibody is raised in rabbits and has high reactivity with human samples, making it suitable for Western blot applications. By binding to the FAS protein, this antibody enables the detection and analysis of FAS expression in various cell types.FAS is a cell surface receptor that plays a key role in programmed cell death, or apoptosis. Dysregulation of the FAS pathway has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
Therefore, understanding the expression and function of FAS is crucial for developing targeted therapies for these conditions.With its specificity and sensitivity, the FAS Polyclonal Antibody (CAB12401) is an essential tool for researchers investigating the apoptotic pathway and its implications in disease. Its reliability and versatility make it an ideal choice for studies in cell biology, immunology, and cancer research.
Product Name:
Fas Rabbit Polyclonal Antibody
SKU:
CAB12401
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-335 of human Fas (NP_000034.1).
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
38kDa
Observed MW:
40-50kDa
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
Purification Method:
Affinity purification
Gene ID:
355
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Fas antibody (CAB12401) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
