The Fas Monoclonal Antibody is a valuable tool for researchers studying apoptosis and cell death mechanisms. This antibody specifically targets Fas, a cell surface receptor that plays a key role in apoptosis signaling pathways. Raised in mice, this antibody is highly specific and reacts with human samples, making it ideal for use in various research applications such as Western blotting and immunohistochemistry. By detecting and analyzing Fas expression in different cell types, researchers can gain insights into the regulatory mechanisms of programmed cell death.
The Fas Monoclonal Antibody is particularly useful for studies in cancer biology, as dysregulation of apoptosis pathways is a hallmark of cancer development. By understanding the role of Fas in promoting or inhibiting cell death, researchers can uncover potential therapeutic targets for cancer treatment.Overall, the Fas Monoclonal Antibody is a valuable tool for researchers investigating cell death mechanisms and exploring novel therapeutic strategies for cancer and other diseases.
Product Name:
Fas Monoclonal Antibody
SKU:
CAB21903
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Fas (NP_000034.1).
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
38kDa
Observed MW:
40-50kDa
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
Purification Method:
Affinity purification
Gene ID:
355
Clone Number:
ARC51709
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse thymus, using Fas Rabbit mAb antibody (CAB21903) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
