The Fas Polyclonal Antibody (CAB0233) is a valuable tool for researchers studying Fas, a cell surface receptor involved in apoptosis and immune responses. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for Western blot applications. By binding to the Fas protein, this antibody allows for the detection and analysis of Fas expression in various cell types, making it ideal for investigations in immunology, cancer research, and other fields where apoptosis and immune regulation are key factors.Fas, also known as CD95, is a crucial mediator of programmed cell death and plays a significant role in immune system function and development. Dysregulation of the Fas signaling pathway has been linked to various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
By studying Fas expression and activity, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new targets for therapeutic intervention.The Fas Polyclonal Antibody (CAB0233) offers researchers a reliable tool for investigating the role of Fas in health and disease. Its specificity, sensitivity, and validated performance in Western blot applications make it a valuable asset for studies aiming to elucidate the molecular mechanisms governing apoptosis, immune responses, and disease pathogenesis.
Product Name:
Fas Rabbit Polyclonal Antibody
SKU:
CAB0233
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human Fas (NP_000034.1).
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
38kDa
Observed MW:
40-50kDa
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
Purification Method:
Affinity purification
Gene ID:
355
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Fas Rabbit pAb (CAB0233) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
