The FAM89B Monoclonal Antibody (PAC039926) is a powerful tool for researchers studying FAM89B, a protein involved in various cellular processes and potential implications in cancer biology. This antibody, produced through a monoclonal antibody development process, exhibits high specificity and sensitivity towards human samples, making it ideal for use in immunofluorescence and immunohistochemistry applications.FAM89B, also known as a member of the FAM89 protein family, has been linked to cellular proliferation, migration, and invasion in cancer cells. The antibody's ability to specifically target FAM89B allows for precise detection and analysis in different cell types, offering valuable insights into the protein's function and potential role in cancer progression.
Research into FAM89B's function is vital for uncovering its mechanisms of action and identifying potential therapeutic targets for cancer treatment. By utilizing the FAM89B Monoclonal Antibody, scientists can further explore the role of this protein in cancer biology and potentially develop new strategies for combating cancer development and progression.
IHC image of PACO39926 diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO39926 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Negatively regulates TGF-beta-induced signaling; in cooperation with SKI prevents the translocation of SMAD2 from the nucleus to the cytoplasm in response to TGF-beta.
Synonyms:
Leucine repeat adapter protein 25, FAM89B, Lrap25
UniProt Protein Function:
FAM89B: Belongs to the FAM89 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Receptor, misc.Chromosomal Location of Human Ortholog: 11q13Cellular Component: cell surface; cytoplasmBiological Process: negative regulation of transforming growth factor beta receptor signaling pathway
