The FAM83G Polyclonal Antibody (PAC059720) is a valuable tool for researchers studying FAM83G, a protein involved in cell proliferation and cancer progression. Produced in rabbits, this antibody exhibits high reactivity with human samples and has been tested for use in Western blot applications. By targeting the FAM83G protein, this antibody enables accurate detection and analysis in a variety of cell types, making it a valuable asset for studies in oncology and cell biology.FAM83G, also known as Family with Sequence Similarity 83 Member G, is a key player in promoting cell growth and division, making it a potential target for cancer therapy research.
Understanding the function of FAM83G is crucial for developing novel treatments for cancer and other diseases related to abnormal cell proliferation. By using the FAM83G Polyclonal Antibody, researchers can gain insights into the role of FAM83G in cancer development and explore its therapeutic potential.
IHC image of PACO59720 diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells with PACO59720 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO59720 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
May regulate the bone morphogenetic proteins (BMP) pathway.
Synonyms:
Protein FAM83G (Protein associated with SMAD1), FAM83G, PAWS1
UniProt Protein Function:
FAM83G: Belongs to the FAM83 family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 17p11.2Cellular Component: cytosol; nucleusMolecular Function: protein bindingBiological Process: BMP signaling pathway
