The FADD Polyclonal Antibody is a valuable tool for researchers studying the Fas-associated death domain-containing protein (FADD), a critical component in the regulation of apoptotic cell death. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding specifically to the FADD protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for investigations in apoptosis, cancer research, and immunology.FADD is a key protein in the extrinsic apoptotic pathway, playing a crucial role in regulating cell death in response to external signals.
Dysregulation of FADD signaling has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions. Understanding the function of FADD is essential for developing targeted therapies to modulate cell death pathways and combat these disease states.In summary, the FADD Polyclonal Antibody offers researchers a reliable tool for studying the role of FADD in cellular processes and disease pathogenesis. Its high reactivity, specificity, and validation for Western blot applications make it a valuable asset for research in molecular biology, cell biology, and disease pathology.
Product Name:
FADD Polyclonal Antibody
SKU:
CAB21699
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human FADD (NP_003815.1).
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
Purification Method:
Affinity purification
Gene ID:
8772
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using FADD Rabbit pAb (CAB21699) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
