The F9 Polyclonal Antibody (CAB1578) offered by Assay Genie is a key tool for researchers studying the F9 protein, which is involved in various cellular processes including cell growth and differentiation. This antibody is produced using a polyclonal approach in rabbits, ensuring high reactivity and specificity for targeting the F9 protein in human samples. Validated for use in Western blot applications, this antibody binds specifically to the F9 protein, allowing for precise detection and analysis in different cell types. Its reliability and sensitivity make it an ideal choice for studies in fields such as developmental biology, stem cell research, and cancer biology where the F9 protein plays a crucial role.
Given the importance of the F9 protein in cell function and disease development, understanding its mechanisms of action is essential for advancing research and potentially developing targeted therapies. By using the F9 Polyclonal Antibody from Assay Genie, researchers can confidently explore the functions of the F9 protein and its implications in various biological processes.
Product Name:
Factor IX/F9 Rabbit Polyclonal Antibody
SKU:
CAB1578
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-192 of human Factor IX / F9 (NP_000124.1).
This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing.
Purification Method:
Affinity purification
Gene ID:
2158
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Factor IX / F9 Rabbit pAb (CAB1578) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
