The F2RL1 Polyclonal Antibody (CAB8796) is a valuable tool for researchers studying F2RL1, also known as protease-activated receptor 2 (PAR-2). This cell surface receptor is involved in key cellular processes such as inflammation, tissue repair, and pain perception. The antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it a reliable choice for experiments involving Western blot analysis.PAR-2 has been implicated in various pathological conditions, including chronic inflammatory diseases and cancer progression.
By targeting this receptor, scientists can gain insights into the molecular mechanisms underlying these diseases and potentially identify novel therapeutic strategies. The F2RL1 Polyclonal Antibody allows for precise detection and quantification of PAR-2 in different cell types, making it an essential tool for researchers in the fields of immunology and oncology.
Product Name:
PAR2 Rabbit Polyclonal Antibody
SKU:
CAB8796
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence within amino acids 15-94 of human PAR2 (NP_005233.4).
HepG2,BxPC-3,Mouse large intestine,Mouse lung,Rat large intestine
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Multi-pass membrane protein.
Calculated MW:
44kDa
Observed MW:
55kDa
This gene encodes a member of the G-protein coupled receptor 1 family of proteins. The encoded cell surface receptor is activated through proteolytic cleavage of its extracellular amino terminus, resulting in a new amino terminus that acts as a tethered ligand that binds to an extracellular loop domain. Activation of the receptor has been shown to stimulate vascular smooth muscle relaxation, dilate blood vessels, increase blood flow, and lower blood pressure. This protein is also important in the inflammatory response, as well as innate and adaptive immunity.
Purification Method:
Affinity purification
Gene ID:
2150
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PAR2 antibody (CAB8796) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
