The F2 Polyclonal Antibody (CAB12382) is a valuable tool for research involving the F2 protein, also known as Coagulation Factor II. This antibody is raised in rabbits and is highly reactive with human samples, making it ideal for studying the role of F2 in coagulation and clot formation.The F2 Polyclonal Antibody is validated for use in Western blot applications, allowing for the detection and analysis of the F2 protein in various cell types. This antibody enables researchers to better understand the function of F2 in hemostasis and thrombosis, providing insights into potential therapeutic strategies for conditions related to blood clotting disorders.
The F2 protein is a key player in the coagulation cascade, making it essential for normal blood clotting. Dysregulation of F2 function can lead to serious health complications, such as excessive bleeding or increased risk of thrombosis. By using the F2 Polyclonal Antibody, researchers can unravel the mechanisms underlying F2 activity and its impact on human health, paving the way for the development of targeted therapies for coagulation disorders.
Product Name:
F2 Rabbit Polyclonal Antibody
SKU:
CAB12382
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 364-622 of human F2 (NP_000497.1).
This gene encodes the prothrombin protein (also known as coagulation factor II). This protein is proteolytically cleaved in multiple steps to form the activated serine protease thrombin. The activated thrombin enzyme plays an important role in thrombosis and hemostasis by converting fibrinogen to fibrin during blood clot formation, by stimulating platelet aggregation, and by activating additional coagulation factors. Thrombin also plays a role in cell proliferation, tissue repair, and angiogenesis as well as maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in this gene lead to various forms of thrombosis and dysprothrombinemia. Rapid increases in cytokine levels following coronavirus infections can dysregulate the coagulation cascade and produce thrombosis, compromised blood supply, and organ failure.
Purification Method:
Affinity purification
Gene ID:
2147
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using F2 Rabbit pAb (CAB12382) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
