The F2 Antibody (CAB12382) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly reactive with human samples, making it ideal for studying the role of F2 in coagulation and clot formation.The F2 Polyclonal Antibody is validated for use in Western blot applications, allowing for the detection and analysis of the F2 protein in various cell types. This antibody enables researchers to better understand the function of F2 in hemostasis and thrombosis, providing insights into potential therapeutic strategies for conditions related to blood clotting disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
F2 Antibody
SKU:
CAB12382
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PT, THPH1, RPRGL2, F2
Positive Sample:
HepG2
Cellular Localization:
Secreted, Extracellular Space.
Calculated MW:
70kDa
Observed MW:
75kDa
This gene encodes the prothrombin protein (also known as coagulation factor II). This protein is proteolytically cleaved in multiple steps to form the activated serine protease thrombin. The activated thrombin enzyme plays an important role in thrombosis and hemostasis by converting fibrinogen to fibrin during blood clot formation, by stimulating platelet aggregation, and by activating additional coagulation factors. Thrombin also plays a role in cell proliferation, tissue repair, and angiogenesis as well as maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in this gene lead to various forms of thrombosis and dysprothrombinemia. Rapid increases in cytokine levels following coronavirus infections can dysregulate the coagulation cascade and produce thrombosis, compromised blood supply, and organ failure.
Purification Method
Affinity purification
Gene ID
2147
RRID
AB_2759226
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using F2 Rabbit pAb (CAB12382) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
