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F10 Rabbit Polyclonal Antibody (CAB15057)

SKU:
CAB15057
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:F10 Rabbit Polyclonal Antibody
SKU:CAB15057
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 41-300 of human F10 (NP_000495.1).
Sequence:ANSF LEEM KKGH LERE CMEE TCSY EEAR EVFE DSDK TNEF WNKY KDGD QCET SPCQ NQGK CKDG LGEY TCTC LEGF EGKN CELF TRKL CSLD NGDC DQFC HEEQ NSVV CSCA RGYT LADN GKAC IPTG PYPC GKQT LERR KRSV AQAT SSSG EAPD SITW KPYD AADL DPTE NPFD LLDF NQTQ PERG DNNL TRIV GGQE CKDG ECPW QALL INEE NEGF CGGT ILSE FYIL TAAH CLYQ AKRF KVRV GDRN TEQE EGGE
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:FX; FXA; F10
Positive Sample:LO2,OVCAR3,mouse heart,mouse liver,rat liver
Conjugate:Unconjugated
Cellular Localization:Secreted.
Calculated MW:55kDa
Observed MW:56kDa

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Purification Method:Affinity purification
Gene ID:2159
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using F10 Rabbit pAb (CAB15057) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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