The F10 Polyclonal Antibody (CAB15057) is a powerful tool for research involving the F10 protein, a crucial component in various cellular processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the F10 protein, this antibody enables accurate detection and analysis in a wide range of cell types, making it an essential tool for studies in molecular biology and cell signaling pathways.The F10 protein, also known as a key player in cell cycle regulation, is involved in processes such as cell growth, proliferation, and apoptosis.
Its role in these fundamental cellular functions makes it a valuable target for research in fields such as cancer biology, developmental biology, and drug discovery. Understanding the mechanisms underlying F10 activity is vital for unraveling its contribution to various diseases and for developing targeted therapies to modulate its functions effectively.
Product Name:
F10 Rabbit Polyclonal Antibody
SKU:
CAB15057
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 41-300 of human F10 (NP_000495.1).
This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.
Purification Method:
Affinity purification
Gene ID:
2159
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using F10 Rabbit pAb (CAB15057) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
