The F10 Polyclonal Antibody (CAB1452) is a valuable tool for researchers studying the F10 protein, a key player in the coagulation cascade. This antibody, generated in rabbits, is highly specific to human samples and is optimized for use in Western blotting applications. By binding to the F10 protein, this antibody allows for the detection and analysis of F10 levels in a variety of cell types, making it ideal for investigations in hemostasis and thrombosis research.
F10, also known as coagulation factor X, is essential for the initiation and propagation of blood clotting. Dysregulation of F10 can lead to bleeding disorders or an increased risk of thrombosis, making it a target of interest for studies on coagulation disorders and cardiovascular diseases. By elucidating the role of F10 in these processes, researchers can uncover potential therapeutic targets for the treatment of clotting disorders and related conditions.
Product Name:
F10 Rabbit Polyclonal Antibody
SKU:
CAB1452
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 235-488 of human F10 (NP_000495.1).
This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.
Purification Method:
Affinity purification
Gene ID:
2159
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using F10 antibody (CAB1452) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
