null

F-TESTO (Free Testosterone) ELISA Kit (AEES00021)

SKU:
AEES00021
Product Type:
ELISA Kit
ELISA Type:
Competitive
Size:
96 Assays
Sensitivity:
18.75 pg/mL
Range:
31.25-2000 pg/mL
Sample Type:
Serum and plasma
$599
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:F-TESTO (Free Testosterone) ELISA Kit
SKU:AEES00021
Target:F-TESTO (Free Testosterone)
Size:96T
Assay type:Competitive-ELISA
Assay time:2.0h
Sensitivity:18.75 pg/mL
Detection range:31.25-2000 pg/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
Micro ELISA Plate (Dismountable)8 wells x 3 strips8 wells x 12 strips-20°C, 6 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent1 vial, 10 mL1 vial, 10 mL4°C (shading light)
Stop Solution1 vial, 10 mL1 vial, 10 mL4°C
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with F-TESTO. During the reaction, F-TESTO in the sample or standard competes with a fixed amount of F-TESTO on the solid phase supporter for sites on the Biotinylated Detection Ab specific to F-TESTO. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of F-TESTO in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)90-9895-10695-106
Average (%)93103101
1:4Range (%)87-9995-10687-100
Average (%)9110193
1:8Range (%)98-10987-10190-104
Average (%)1059497
1:16Range (%)96-10482-9592-105
Average (%)1009099
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)94-10599
EDTA plasma (n=5)96-107103
Cell culture media (n=5)88-10194
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n2020.020.020.020.020.0
Mean (ng/mL)98.04216.36884.0587.82219.17800.62
Standard deviation4.9710.1746.594.5110.7642.43
C V (%)5.074.75.275.144.915.3
Sample type &Sample volume:serum and plasma; 50μL
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of F-TESTO concentrations in serum and plasma.
Specificity:This kit recognizes F-TESTO in samples. No significant cross-reactivity or interference between F-TESTO and analogues was observed.

Related Products