The EYA3 Polyclonal Antibody (CAB15056) is a crucial tool for researchers studying the EYA3 protein, which is involved in a variety of cellular processes including DNA repair, cell cycle control, and development. This antibody, produced in rabbits, has been validated for use in Western blot applications and demonstrates high reactivity with human samples.EYA3 is a member of the EYA family of proteins, known for their role in mediating protein-protein interactions and acting as transcriptional co-activators.
EYA3 specifically has been linked to various pathways related to cancer development, making it a promising target for cancer research.By using the EYA3 Polyclonal Antibody, researchers can detect and analyze the expression of EYA3 in different cell types, providing valuable insights into its function and potential therapeutic implications. This antibody is an essential tool for studies in cancer biology, developmental biology, and DNA damage response pathways.
Product Name:
EYA3 Rabbit Polyclonal Antibody
SKU:
CAB15056
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EYA3 (NP_001981.2).
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2140
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EYA3 antibody (CAB15056) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
